bglH

168

phospho-beta-glucosidase

Locus: BSU_39260

Molecular weight: 53.12 kDa

Isoelectric point: 4.96

Protein length: 469 aa

Gene length: 1410 bp

Function: salicin utilization

Product: phospho-beta-glucosidase

Essential: no

E.C.: 3.2.1.86

Synonyms: bglH

Genomic Context

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List of homologs in different organisms, belongs to COG2723 (Galperin et al., 2021)

Gene

Coordinates: 4,032,346  4,033,755

The protein

Catalyzed reaction/ biological activity

• 6-phospho-β-D-glucosyl-(1→4)-D-glucose + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• glycosyl hydrolase 1 family (according to UniProt)

Structure

• 4IPL (PDB) (from Streptococcus pneumoniae, 60% identity) PubMed
• P40740 (AlphaFold)

Expression and Regulation

Operons

• Genes: bglP-bglH-yxiE

  Description: PubMed

  Regulation

  • induced by salicin (LicT) PubMed

  Regulatory mechanism

  • CcpA: repression, (CcpA binding site overlaps -35 region) PubMed, in ccpA regulon
  • LicT: anti-termination, via LicT-dependent RNA switch, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HprK, in licT regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • An ncRNA is predicted for 'BglP' PubMed

Biological materials

Mutant

• BKE39260 (bglH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATCACCCCATTAG,  downstream forward: _UP4_TGACAATTTTTTGAAAACTC
• BKK39260 (bglH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATCACCCCATTAG,  downstream forward: _UP4_TGACAATTTTTTGAAAACTC

References

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