bglA

168

6-phospho-beta-glucosidase

locus: BSU_40110

Molecular weight: 54.67 kDa

pI: 4.65

Protein length: 479 aa

Gene length: 1440 bp

function: beta-glucoside utilization

product: 6-phospho-beta-glucosidase)

essential: no

ec: 3.2.1.86

synonyms: bglA

List of homologs in different organisms, belongs to COG2723 (Galperin et al., 2021)

Gene

Coordinates: 4,121,166  4,122,605

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The protein

Catalyzed reaction/ biological activity

• 6-phospho-β-D-glucosyl-(1→4)-D-glucose + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• [wiki|glycosyl hydrolase 1 family] (according to UniProt)

Structure

• [PDB|2XHY] (from E. coli, 66% identity) [pubmed|22393408]
• [AF|P42973]

Expression and Regulation

Operons

• genes: [gene|FF3C9A092A3CAE0EB808F9C615BF5FC433C17E81|yyzE]-[gene|6A06E0E3017DB6E75D5C1114D954D51CED546051|bglA]

  description: [pubmed|22383849]

Biological materials

Mutant

• BKE40110 ([gene|6A06E0E3017DB6E75D5C1114D954D51CED546051|bglA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCATGTTTAATT,  downstream forward: _UP4_TAAGAAGCGCTCGAATCGAG
• BKK40110 ([gene|6A06E0E3017DB6E75D5C1114D954D51CED546051|bglA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCATGTTTAATT,  downstream forward: _UP4_TAAGAAGCGCTCGAATCGAG

References

• 14652714,15139916,10627040,8125345