bdbA

168

thiol-disulfide oxidoreductase

locus: BSU_21460

Molecular weight: 16.04 kDa

pI: 8.34

Protein length: 137 aa

Gene length: 414 bp

function: oxidative folding of proteins

product: thiol-disulfide oxidoreductase

essential: no

synonyms: bdbA, yolI

List of homologs in different organisms, belongs to COG3118 (Galperin et al., 2021)

Gene

Coordinates: 2,266,936 → 2,267,349

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The protein

Protein family

• [wiki|Thioredoxin family] (according to UniProt)

[wiki|Domains]

• [wiki|Thioredoxin domain] (aa 26-136) (according to UniProt)

Structure

• [AF|P68569]

[wiki|Localization]

• secreted (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|1A6D90298D039FFFD977B2534952BA5E32B3530F|sunA]-[gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]-[gene|DB5F719C0A2F610301E174F6890DA9E826C76932|bdbA]-[gene|6B2DC9873B7B693D9D0FC0D773740140D0640C9E|sunS]-[gene|12CE5AE0BD02C989E803480A1CE5A47D6192D751|bdbB]

  description: [Pubmed|22383849]

  regulation

  • repressed by [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]-[wiki|DnaA] [Pubmed|27902860,15743949]
  • expression starts in the stationary phase [pubmed|30808982]
  • expression is heterogeneous in the population, this is mediated by [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB], [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok], and [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] [pubmed|30808982]

  regulatory mechanism

  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|15743949], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]: activation, [Pubmed|20817675,19465659], in [regulon|protein:5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|27902860,15743949], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]: activation, [Pubmed|16306698], in [regulon|protein:7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675,17720793], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  additional information

  • the mRNA is very stable (> 15 min) [pubmed|12884008]
  • the amount of the mRNA is substantially decreased upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• BKE21460 (Δ[gene|DB5F719C0A2F610301E174F6890DA9E826C76932|bdbA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATATAGAATACTCCTT,  downstream forward: _UP4_TTTGATAAAAATGGTGATAG
• BKK21460 (Δ[gene|DB5F719C0A2F610301E174F6890DA9E826C76932|bdbA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATATAGAATACTCCTT,  downstream forward: _UP4_TTTGATAAAAATGGTGATAG

References

• 27902860,11872755,15743949,21815947,20817675,28698374