spsB

168

putative glycosyltransferase

Locus: BSU_37900

Molecular weight: 54.16 kDa

Isoelectric point: 5.04

Protein length: 474 aa

Gene length: 1425 bp

Function: spore crust polysaccharide synthesis

Product: putative glycosyltransferase

Essential: no

Synonyms: spsB, ipa-64d

Genomic Context

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List of homologs in different organisms, belongs to COG1887 (Galperin et al., 2021)

Gene

Coordinates: 3,890,922  3,892,346

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed
• less hydrophobic spore surface PubMed

The protein

Structure

• P39622 (AlphaFold)

Localization

• outer spore coat protein, localization requires CotE PubMed

Expression and Regulation

Operons

• Genes: spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE37900 (spsB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTGCTTAAATAGGCCGACA,  downstream forward: _UP4_TAAAATGATAGGAGCGCAAA
• BKK37900 (spsB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTGCTTAAATAGGCCGACA,  downstream forward: _UP4_TAAAATGATAGGAGCGCAAA

References

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