cotI

168

outer spore coat protein

Locus: BSU_30920

Molecular weight: 41.09 kDa

Isoelectric point: 4.99

Protein length: 357 aa

Gene length: 1074 bp

Function: spore envelope

Product: outer spore coat protein

Essential: no

Synonyms: cotI, ytaA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5881 (Galperin et al., 2021)

Gene

Coordinates: 3,162,084 → 3,163,157

The protein

Protein family

• cotS family (with CotS and YutH, according to UniProt)

Structure

• 2Q83 (PDB) PubMed
• O34656 (AlphaFold)

Localization

• outer spore coat PubMed

Expression and Regulation

Operons

• Genes: cotI

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-A030 (ytaA::erm), available at the NBRP B. subtilis, Japan
• BKE30920 (ΔcotI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTTACCTGTTTTACTC,  downstream forward: _UP4_TAAGACCATCATTTCCCCCG
• BKK30920 (ΔcotI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTTACCTGTTTTACTC,  downstream forward: _UP4_TAAGACCATCATTTCCCCCG

References

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