rqcU

168

ribosome quality control (RQC) upstream factor, senses collisions between stalled and translating ribosomes, splits stalled ribosomes and targets the nascent peptide for degradation by the ribosome quality control pathway

locus: BSU_28580

Molecular weight: 87.23 kDa

pI: 5.79

Protein length: 785 aa

Gene length: 2358 bp

function: initiation of ribosome-associated translation quality control

product: ribosome quality control (RQC) upstream factor

essential: no

synonyms: mutSB, yshD, mutS2

List of homologs in different organisms, belongs to COG1193 (Galperin et al., 2021)

Gene

Coordinates: 2,920,936 → 2,923,293

Phenotypes of a mutant

• hypersensitive to antibiotics that target ribosomes [pubmed|37205477]
• enhanced formation of [gene|F1969E4C7BAAF70BCBE570F58350C12A3E417539|rpsB] or [gene|144D952B05EBBFF41EC92DF906543EA00475FE69|rpsE] suppressor mutants after mitomycin treatment [pubmed|34339280]

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The protein

Catalyzed reaction/ biological activity

• ATPase activity: ATP + H2O --> ADP + H+ + phosphate [pubmed|38177497,37205477]
• splits stalled ribosomes and targets the nascent peptide for degradation by the ribosome quality control pathway [pubmed|38177497,37205477]

Protein family

• DNA mismatch repair mutS family (with [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS], according to UniProt)

[wiki|Domains]

• Smr domain (aa 710-785) (according to UniProt)

Structure

• [AF|P94545]

Expression and Regulation

Operons

• genes: [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]-[gene|93726C423313C653C4F71CB107FA12F686959E41|yshB]-[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]-[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]

  description: [Pubmed|22383849]

  regulation

  • constitutively expressed [Pubmed|23701187]

Biological materials

Mutant

• MGNA-B007 (yshD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1006 NBRP B. subtilis, Japan]
• 1A845 ( ''mutSB''::''cat''), [Pubmed|11254128], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A845&Search=1A845 BGSC]
• 1A845 ( ''mutSB''::''cat''), [Pubmed|11254128], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A845&Search=1A845 BGSC]
• BKE28580 (Δ[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGATTGTGTGAGCCTCCT,  downstream forward: _UP4_CTAAAATAAAAGGGAGTATG
• BKK28580 (Δ[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGATTGTGTGAGCCTCCT,  downstream forward: _UP4_CTAAAATAAAAGGGAGTATG

labs

• [wiki|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]

References

Reviews

• 22933559

Original Publications

• 11254128,27799325,30814990,34339280,35264791,37205477,38177497