yonN

168

SP prophage-derived DNA-binding protein HU 2

locus: BSU_21050

Molecular weight: 9.71 kDa

pI: 10.23

Protein length: 92 aa

Gene length: 279 bp

function: untwisting of the DNA double helix

product: DNA-binding protein HU 2

essential: no

synonyms: yonN

List of homologs in different organisms, belongs to COG0776 (Galperin et al., 2021)

Gene

Coordinates: 2,225,337  2,225,615

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The protein

Catalyzed reaction/ biological activity

• [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth] endonuclease activity at AP sites [Pubmed|21954439]

Protein family

• bacterial histone-like protein family (with [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs], according to UniProt)

Structure

• [PDB|1HUE] ([protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs] from B. stearothermophilus, 71% identity) [pubmed|7500343]
• [AF|P68573]

Paralogous protein(s)

• [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs]

Expression and Regulation

Operons

• genes: [gene|121C3C9CF49711316A526B9571F020BE8E304B31|yonP]-[gene|34380A2F469ACFD90D450717A41D6F5CC19DCD7E|yonO]-[gene|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]

  description: [pubmed|22383849]

Biological materials

Mutant

• BKE21050 ([gene|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCAATTTCCTCCTAA,  downstream forward: _UP4_TAATCAGTTAATGAGGACGA
• BKK21050 ([gene|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCAATTTCCTCCTAA,  downstream forward: _UP4_TAATCAGTTAATGAGGACGA

References

• 21954439,7500343