defB

defB
168

N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine

locus
BSU_14560
Molecular weight
20.51 kDa
pI
5.46
Protein length
Gene length
function
utilization of S-methyl-cysteine
product
N-formylcysteine deformylase
essential
no
ec
3.5.1.88
synonyms
defB, ykrB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0242 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,526,195  1,526,749
Phenotypes of a mutant
a ''[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA] [gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' double mutant is not viable unless the cells acquire suppressor mutations in ''[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt], [gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]'' or ''[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]'' [Pubmed|27983482]
no growth with S-methyl cysteine [Pubmed|23944997]
inactivation of ''[gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' reduces sporulation efficiency to 9% that of wild type cells; engulfment and fission defects, with reduced [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activity [Pubmed|26735940]
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The protein
Catalyzed reaction/ biological activity
N-acetylcysteine - cysteine + acetate [Pubmed|23944997]
H2O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)
Protein family
polypeptide deformylase family (with [protein|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA], according to UniProt)
Structure
[PDB|1LQY] (complex with antibiotic actinonin, Geobacillus stearothermophilus, 67% identity) [pubmed|12126617]
[AF|Q45495]
Paralogous protein(s)
[protein|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]
Expression and Regulation
Operons
genes
[gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]
description
[pubmed|22383849]
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[gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]

2024-02-03 01:12:42

Jstuelk

34

25e4ac254d10db0959d4e720b04c93c280201f3f

79FAAC433B03035AAA5B10BAFB040F6C3DBA55F9

Biological materials
Mutant
MGNA-A901 (ykrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/901 NBRP B. subtilis, Japan]
BKE14560 ([gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA,  downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC
BKK14560 ([gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA,  downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC
References
11429456,23944997,26735940,27983482,12126617

53B760316B19E142FC3ECAD2CFAA540377B08BBC

Page visits: 3900

Time of last update: 2024-03-28 20:03:41

Author of last update: Melvin.boenninger