kinA

kinA
168

two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay

Locus
BSU_13990
Molecular weight
68.99 kDa
Isoelectric point
5.49
Protein length
Gene length
Function
initiation of sporulation
Product
two-component sensor kinase
Essential
no
Synonyms
kinA, spoIIF, spoIIJ, gsiC, scoD

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5805 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,470,026 → 1,471,846
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of Spo0F
mainly active in the older, inner regions of a colony (with KinB) PubMed
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
three tandem PAS domains in the N-terminal region of KinA, the second PAS domain is the major N-terminal determinant of KinA dimerization PubMed
the first PAS domain is required for NAD(+) binding PubMed
3 PAS domains (aa 3-73, aa 140-214, aa 265-335) (according to UniProt)
2 PAC domains (aa 77-116, aa 218-255) (according to UniProt)
Histidine Kinase domain (aa 402-606) (according to UniProt)
Structure
Modification
autophosphorylation on a His residue
Effectors of protein activity
interaction with PxpB or Sda inhibits autophosphorylation of KinA PubMed
autophosphorylation is inhibited by SivA and BslA PubMed
activated by interaction with NAD(+) that indicated poor respiratory activity of the cell PubMed, this has been falsified PubMed
cytoplasm
Expression and Regulation
Operons
Genes
Description
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
stringent response: positive regulation, in stringent response
Sigma factors
SigH: sigma factor, PubMed, in sigH regulon
Additional information
translation is repressed upon interaction of the mRNA with the sr1 RNA PubMed
promoter-up mutations or high artificial expression that render ''kinA'' independent from SigH, allow sporulation at high salinity PubMed
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kinA

2025-01-18 00:14:27

Jstuelk

112

0E62F7AD8CD3091271F54BC16A545F920A2475EE

E1D98A4CF39241213C2BB7A9EEEA407E06CC2045

Biological materials
Mutant
BKE13990 (ΔkinA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CACAGAATCCCTCCTTTGCA,  downstream forward: _UP4_TTTCCAAAAAAATAAAAACA
BKK13990 (ΔkinA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CACAGAATCCCTCCTTTGCA,  downstream forward: _UP4_TTTCCAAAAAAATAAAAACA
References
Reviews
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Original Publications
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B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2

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Time of last update: 2025-01-18 00:11:59

Author of last update: Jstuelk