motB

168

H⁺-coupled MotA-MotB flagellar stator

Locus: BSU_13680

Molecular weight: 29.33 kDa

Isoelectric point: 6.65

Protein length: 261 aa

Gene length: 786 bp

Function: Motility and chemotaxis

Product: flagellar stator subunit

Essential: no

Synonyms: motB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1360 (Galperin et al., 2021)

Gene

Coordinates: 1,433,676 → 1,434,461

Phenotypes of a mutant

• increased DegU-P formation, resulting in reduced comK expression and strongly reduced genetic competence PubMed
• loss of swimming and Swarming motility PubMed
• mucoid phenotype due to the DegU-P activated overexpression of the ''capB-capC-capA-capE'' operon and resulting overproduction of poly-gamma-glutamate PubMed

The protein

Protein family

• MotB family (with MotS, according to UniProt)

Structure

• 6YSF (the MotA₅-MotB₂ complex from Clostridium sporogenes) PubMed
• 4B62 (PDB) (from Pseudomonas aeruginosa, corresponds to aa 110 ... 255, 33% identity)
• P28612 (AlphaFold)

Modification

• protonation of Asp-24 is essential for torque generation PubMed

Paralogous protein(s)

• MotS

Localization

• anchored to the cell wall, extending through the cell membrane PubMed

Expression and Regulation

Operons

• Genes: motA-motB

  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• available in Nicola Stanley-Wall's lab PubMed
• BKE13680 (ΔmotB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCTCATGCTTCTTCTTCTTT,  downstream forward: _UP4_TAGCAAAAAAGGAAGCCTTG
• BKK13680 (ΔmotB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCTCATGCTTCTTCTTCTTT,  downstream forward: _UP4_TAGCAAAAAAGGAAGCCTTG

References

Reviews

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Original Publications

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