ssuB

168

aliphatic sulfonate ABC transporter (ATP-binding protein)

Locus: BSU_08830

Molecular weight: 30.08 kDa

Isoelectric point: 8.67

Protein length: 255 aa

Gene length: 768 bp

Function: sulfonate uptake

Product: aliphatic sulfonate ABC transporter (ATP-binding protein)

Essential: no

Synonyms: ssuB, ygaL

Genomic Context

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List of homologs in different organisms, belongs to COG1116 (Galperin et al., 2021)

Gene

Coordinates: 961,394  962,161

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 7-231) (according to UniProt)

Structure

• 1Q1B (PDB) (MalK from E. coli, 38% identity) PubMed
• P97027 (AlphaFold)

Paralogous protein(s)

• YtlC

Localization

• associated to the membrane (via SsuC) PubMed

Expression and Regulation

Operons

• Genes: ssuB-ssuA-ssuC-ssuD-ygaN

  Description: PubMed

  Regulation

  • repressed in the presence of cysteine (CymR) PubMed

  Regulatory mechanism

  • CymR: repression, PubMed, in cymR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A249 (ygaL::erm), available at the NBRP B. subtilis, Japan
• BKE08830 (ssuB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC,  downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG
• BKK08830 (ssuB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC,  downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG

References

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