adaA

168

trigger enzyme

Locus: BSU_01810

Molecular weight: 24.15 kDa

Isoelectric point: 9.19

Protein length: 211 aa

Gene length: 636 bp

Function: adaptive response to alkylative DNA damage

Product: trigger enzyme

Essential: no

Synonyms: adaA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2169 (Galperin et al., 2021)

Gene

Coordinates: 203,729  204,364

Phenotypes of a mutant

• enhanced formation of rpsB or rpsE suppressor mutants after mitomycin treatment PubMed

The protein

Catalyzed reaction/ biological activity

• methylphosphotriester deoxyribonucleoside in DNA + L-cysteinyl-[protein] --> deoxyribonucleotide in DNA + H⁺ + S-methyl-L-cysteinyl-[protein] (according to UniProt)

Protein family

• AraC family

Domains

• HTH araC/xylS-type domain (aa 102-200) (according to UniProt)

Structure

• 1ZGW (PDB) (from E. coli, 39% identity) PubMed
• P19219 (AlphaFold)

Expression and Regulation

Operons

• Genes: adaA-adaB

  Description: PubMed

  Regulation

  • positive control by AdaA PubMed

  Regulatory mechanism

  • AdaA: activation, in adaA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE01810 (adaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATCTCACCTCTCCTT,  downstream forward: _UP4_ATGAGTAAAATGGAGGAAAC
• BKK01810 (adaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATCTCACCTCTCCTT,  downstream forward: _UP4_ATGAGTAAAATGGAGGAAAC

References

Reviews

Loading

Original Publications

Loading