ispF

168

2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis

locus: BSU_00910

Molecular weight: 16.98 kDa

pI: 5.4

Protein length: 158 aa

Gene length: 477 bp

function: MEP pathway of isoprenoid biosynthesis

product: 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase

essential: yes

ec: 4.6.1.12

synonyms: ispF, yacN

List of homologs in different organisms, belongs to COG0245 (Galperin et al., 2021)

Gene

Coordinates: 110,480  110,956

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• 4-CDP-2-C-methyl-D-erythritol 2-phosphate --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP (according to UniProt)

Protein family

• IspF family (single member, according to UniProt)

Structure

• [PDB|5IWX] (native) [Pubmed|29335298]
• [PDB|5IWY] (complex with CMP) [Pubmed|29335298]
• [AF|Q06756]

Biological materials

Mutant

• MGNA-B882 (yacN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1881 NBRP B. subtilis, Japan]

References

• 15805528,10482513,12270818,17458547,23840410,15502301,29335298