ctsR

ctsR
168

transcription repressor of class III heat shock genes ([gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] operon, [gene|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE], [gene|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP])

locus
BSU_00830
Molecular weight
17.60 kDa
pI
9.26
Protein length
Gene length
function
regulation of protein degradation
product
transcription repressor
essential
no
synonyms
ctsR, yacG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4463 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
101,449  101,913
Phenotypes of a mutant
reduced expression of [gene|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB], due to reduced [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] activity [pubmed|30962353]
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The protein
Protein family
CtsR family (single member, according to UniProt)
Structure
[PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]
[PDB|6FH4] (C-terminal domain bound to phosphoarginine) [pubmed|30962626]
[AF|P37568]
Modification
phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]) [Pubmed|27749819]
phosphorylation of a tyrosine residue by [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] [Pubmed|14984053]
recently, it was reported that CtsR is phosphorylated by [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]
in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]
Effectors of protein activity
CtsR is inactivated by heat, heat sensing requires Gly-64 [Pubmed|20852588]
non-phosphorylated [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] targets CtsR for degradation [Pubmed|20852588]
regulated proteolysis by [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]/[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] [Pubmed|11179229,16163393,17380125]
Expression and Regulation
Operons
genes
[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]-[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[gene|151F226370D225776F3FE7EA4901485095F1AC45|radA]-[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]
description
[Pubmed|8793870]
regulation
expressed during germination and spore outgrowth [Pubmed|24244006]
regulatory mechanism
[protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]: repression, [pubmed|9987115,11179229,16163393,17380125], in [regulon|protein:908DB17A39D518E84977250C55825E77FA02E391|ctsR regulon]
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|30962353], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8793870], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [pubmed|8793870] [pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
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[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]→[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]

2024-03-11 10:30:02

Bzhu

103

b7066b5ca5004b095502d71420c469e9ee925bfa

163769A1708F45F8ABE642374A97FA8A00293529

additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
GP3202([gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]::''ermC''), available in [wiki|Jörg Stülke]'s lab
MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]
''ctsR::aphA3'' availbale in [wiki|Ulf Gerth]'s lab
BKE00830 (''[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]''::''erm'', available in the BGSC and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
BKE00830 ([gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC,  downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
BKK00830 ([gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC,  downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [wiki|Ulf Gerth]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP3798 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Ulf Gerth]'s lab
References
Reviews
14984053,21078442,23375660,27518094,28402413,28748186,34438398,37251512
Original Publications
27749819,30962626,30962353,11179229,20852588,37946188

908DB17A39D518E84977250C55825E77FA02E391

Page visits: 7520

Time of last update: 2024-03-28 09:56:05

Author of last update: Dennis.wicke