pabC

168

aminodeoxychorismate lyase

locus: BSU_00760

Molecular weight: 33.36 kDa

pI: 6.12

Protein length: 293 aa

Gene length: 882 bp

function: biosynthesis of folate

product: aminodeoxychorismate lyase

essential: no

ec: 4.1.3.38

synonyms: pabC

List of homologs in different organisms, belongs to COG0115 (Galperin et al., 2021)

Gene

Coordinates: 84,874  85,755

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The protein

Catalyzed reaction/ biological activity

• 4-amino-4-deoxychorismate --> 4-aminobenzoate + pyruvate (according to UniProt)

Protein family

• [wiki|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• PLP

Structure

• [PDB|4WHX] (from ''B. pseudomallus'', 27% identity)
• [AF|P28821]

[wiki|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|B189AD3E3D18876E1956763182D835A151727392|pabB]-[gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC]-[gene|FA9539CB701BC47FD4E849FBA6D7B201B27C6104|sul]-[gene|AAE44B4F73C7C02F7DB3574B6169E0DE46F4B787|folB]-[gene|D2B189C6292ACE1B25658B407C284E9A683EAE5B|folK]-[gene|F373EBC35805DC551E8AFF6FDC7E43D5587C4700|yazB]-[gene|CE45D56BC413555AF4E7914B5307AD967A358452|yacF]-[gene|24CC89DE55B3C19F80B11D106B9FD241174AE3E4|lysS]

  description: [Pubmed|9084182]

  regulation

  • translation repressed by tryptophan ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|9084182]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9084182], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

additional information

• the [gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC] part of the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• BKE00760 ([gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATACCGGCCGTTCACATATA,  downstream forward: _UP4_ATTCATGAAAAGGGAGGAAG
• BKK00760 ([gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATACCGGCCGTTCACATATA,  downstream forward: _UP4_ATTCATGAAAAGGGAGGAAG

References

• 9084182,21815947