yacD

168

putative peptidyl-prolyl cis-trans isomerase, similar to secretion protein [protein|6EE13E61A218A6D52BAC83A1145B0B96961CE289|prsA]

locus: BSU_00720

Molecular weight: 33.94 kDa

pI: 4.95

Protein length: 297 aa

Gene length: 894 bp

function: unknown

product: unknown

essential: no

synonyms: yacD

List of homologs in different organisms, belongs to COG0760 (Galperin et al., 2021)

Gene

Coordinates: 80,802  81,695

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The protein

Catalyzed reaction/ biological activity

• [protein]-peptidylproline (ω=180) --> [protein]-peptidylproline (ω=0) (according to UniProt)
• peptidyl-prolyl cis-trans isomerase activity (according to UniProt)

[wiki|Domains]

• PpiC domain (aa 154-247) (according to UniProt)

Structure

• [PDB|5EZ1] (from Helicobacter pylori, 26% identity) [pubmed|26850168]
• [AF|P37566]

[wiki|Localization]

• extracellular (with signal peptide) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]-[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]-[gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]-[gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK]

  description: [Pubmed|22383849]

  regulation

  • induced by heat stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|spx]) [pubmed|30480837]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|30480837], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]

Biological materials

Mutant

• MGNA-B925 (yacD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1924 NBRP B. subtilis, Japan]
• BKE00720 ([gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAACCTGCTTTCAGCCCCT,  downstream forward: _UP4_TGATTGACAAAAAATTTTGA
• BKK00720 ([gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAACCTGCTTTCAGCCCCT,  downstream forward: _UP4_TGATTGACAAAAAATTTTGA

References

• 36815589