ctc

168

general stress protein, non-essential ribosomal protein BL25

locus: BSU_00520

Molecular weight: 21.91 kDa

pI: 4.22

Protein length: 204 aa

Gene length: 615 bp

function: [wiki|translation] (under stress conditions)

product: ribosomal protein BL25

essential: no

synonyms: ctc, rplY

List of homologs in different organisms, belongs to COG1825 (Galperin et al., 2021)

Gene

Coordinates: 58,783  59,397

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The protein

Protein family

• Bacterial ribosomal protein bL25 family (single member, according to UniProt)

Structure

• [PDB|1FEU] (from ''Thermus thermophilus'', 32% identity, 53% similarity) [Pubmed|11418764]
• [AF|P14194]

[wiki|Localization]

• in the ribosome (large subunit) [Pubmed|12432960]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

additional information

• translation is substantially enhanced when the gene is transcribed by [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[wiki|RNA polymerase] [pubmed|33927010]

Biological materials

Mutant

• MGNA-B912 (ctc::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1911 NBRP B. subtilis, Japan]
• GP500(spc), available in [wiki|Jörg Stülke]'s lab [Pubmed|12432960]
• CS206 (''[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]''::''aphA3'', available in [wiki|Colin Harwood]'s and [wiki|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE00520 ([gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCACCATCCTCT,  downstream forward: _UP4_TAATAGCTTAAGGCGTAACC
• BKK00520 ([gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCACCATCCTCT,  downstream forward: _UP4_TAATAGCTTAAGGCGTAACC

Expression vectors

• expression/ purification with N-terminal His-tag in ''E. coli'': pGP804 (in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab [Pubmed|12432960]

Antibody

• available in [wiki|Jörg Stülke]'s lab [Pubmed|12432960]

References

Reviews

• 19216708

Original Publications

• 2836704,3123466,3100810,8522540,15805528,12432960,6790515,2836704,116131,11418764,23002217,27197833,33753464,33927010