yaaK

168

similar to DNA-binding protein

locus: BSU_00200

Molecular weight: 11.64 kDa

pI: 4.54

Protein length: 107 aa

Gene length: 324 bp

function: unknown

product: unknown

essential: no

synonyms: yaaK

List of homologs in different organisms, belongs to COG0718 (Galperin et al., 2021)

Gene

Coordinates: 28,529  28,852

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The protein

Protein family

• YbaB/EbfC family (single member, according to UniProt)

Structure

• [PDB|1YBX] (from Clostridium thermocellum, 53% identity)
• [AF|P24281]

[wiki|Localization]

• nucleoid (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|73D063EDC412F7F28DA27C986ECCCD1C4A1F6618|dnaX]-[gene|9AFCEFE83560B1E2B1FFD7CFD2E4F17FAE137889|yaaK]-[gene|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR]-[gene|C5127208E7562F09249112240F0BE8C582B2E5AD|yaaL]-[gene|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|bofA]

  description: [Pubmed|22383849]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• MGNA-B892 (yaaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1891 NBRP B. subtilis, Japan]
• BKE00200 ([gene|9AFCEFE83560B1E2B1FFD7CFD2E4F17FAE137889|yaaK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCATTCACTCTCTTTC,  downstream forward: _UP4_TTATTCTAGGGGGATAAAAG
• BKK00200 ([gene|9AFCEFE83560B1E2B1FFD7CFD2E4F17FAE137889|yaaK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCATTCACTCTCTTTC,  downstream forward: _UP4_TTATTCTAGGGGGATAAAAG

References

Reviews

• 36882621

Original Publications

• 12884008,19594923