atpI

168

ATP synthase (subunit i)

locus: BSU_36880

Molecular weight: 14.35 kDa

pI: 10.16

Protein length: 127 aa

Gene length: 384 bp

function: ATP synthesis

product: ATP synthase (subunit i))

essential: no

synonyms: atpI

List of homologs in different organisms

Gene

Coordinates: 3,787,620  3,788,003

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The protein

Catalyzed reaction/ biological activity

• ATP synthesis [http://www.youtube.com/watch?v=uOoHKCMAUMc see a video]

Protein family

• bacterial atpI family (single member, according to UniProt)

Structure

• [AF|P37816]

Effectors of protein activity

• ATPase activity is inhibited upon binding of Mg-ADP to [protein|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD] [pubmed|30580998]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI]-[gene|CC1894D90AC17487A286B2909964916825055F93|atpB]-[gene|6CF7F6303425D8A51E1E01BF18823139E4259A8F|atpE]-[gene|87311FEA265AD3F5526C78D631E7E51BBF01F0D6|atpF]-[gene|E956A30D97B2941108880F3AD48620C4DB6E0EBD|atpH]-[gene|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]-[gene|492EEC8068043DA770F5DC1D54799C4366AE9A01|atpG]-[gene|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD]-[gene|A9DA64F8C7A60E8E2B1EDC99BEA1BD7DD0135AA6|atpC]

  description: [Pubmed|9353933]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the mRNA is processed between [gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI] and [gene|CC1894D90AC17487A286B2909964916825055F93|atpB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

Biological materials

Mutant

• BKE36880 ([gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAATTATCTGTCTCCTG,  downstream forward: _UP4_TCAATGGAAGAGAGGTGAAA
• BKK36880 ([gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAATTATCTGTCTCCTG,  downstream forward: _UP4_TCAATGGAAGAGAGGTGAAA

References

• 7961438,29794222,30580998