aspB

168

aspartate transaminase

locus: BSU_22370

Molecular weight: 42.93 kDa

pI: 5.3

Protein length: 393 aa

Gene length: 1182 bp

function: biosynthesis of aspartate

product: aspartate transaminase

essential: no

ec: 2.6.1.1

synonyms: aspB

List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

Gene

Coordinates: 2,347,660 2,348,841

Phenotypes of a mutant

• auxotrophic for aspartate and asparagine [pubmed|38483038,29995990], the auxotrophy for aspartate can be bypassed by derepression of the [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB] operon (inactivation of [gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [pubmed|38483038]
• lyses on DSM dporulation medium due to the inhibition of GltT-mediated Asp transport by glutamate and resulting depletion of meso-diaminopimelate [pubmed|29995990]

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The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + L-aspartate --> L-glutamate + oxaloacetate (according to UniProt)

Protein family

• [wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• PLP

Structure

• [PDB|1J32] (from ''Phormidium lapideum'', 45% identity, 66% similarity)
• [AF|P53001]

Paralogous protein(s)

• [protein|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT], [protein|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|patA]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|A0001681244113B808757281C1B70C0C94F28A27|panB]-[gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]-[gene|EE712C1EC9E9C371847C83F7D75C189B276CD4B6|panD]-[gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]-[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]

  description: [Pubmed|22383849]

• genes: [gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]

  description: [Pubmed|29995990,22383849]

• genes: [gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]

  description: [Pubmed|22383849]

  regulation

  • constitutive

Biological materials

Mutant

• GP4250 (Δ[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::''neo''), available in [wiki|Jörg Stülke]'s lab
• BKE22370 ([gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT
• BKK22370 ([gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT

References

• 15378759,28232482,26883633,29995990,38483038