asnB

asnB
168

asparagine synthetase (glutamine-hydrolysing), amidates m-diaminopimelic acid

locus
BSU_30540
Molecular weight
72.49 kDa
pI
5.61
Protein length
Gene length
function
control of peptidoglycan hydrolysis
product
asparagine synthetase (glutamine-hydrolysing)
essential
yes
ec
6.3.5.4
synonyms
asnB, asn

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0367 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,125,777  3,127,675
Phenotypes of a mutant
the mutant requires excess Mg2+ for growth [pubmed|28317238]
the mutant id unable to grow on LB medium, but grows on minimal medium (MSgg) [pubmed|36069446]
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The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-aspartate + L-glutamine --> AMP + diphosphate + H+ + L-asparagine + L-glutamate (according to UniProt)
amidation of meso-diaminopimelic (mDAP) acid in peptidoglycan [pubmed|28317238]
Protein family
asparagine synthetase family (with [protein|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO] and [protein|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH], according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-2 domain] (aa 2-214) (according to UniProt)
Structure
[PDB|1CT9] (from ''E. coli'', 29% identity) [Pubmed|10587437]
[AF|P54420]
Expression and Regulation
Operons
genes
[gene|52D4DA0AA45F0774543786B55D18DF9DF53A5029|metK]-[gene|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB]-[gene|219586A3F378DC38EA076FD39B99D41136BDE722|alaP]
description
[Pubmed|33155333]
regulation
constitutive
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[gene|52D4DA0AA45F0774543786B55D18DF9DF53A5029|metK]→[gene|219586A3F378DC38EA076FD39B99D41136BDE722|alaP]

2024-03-22 02:17:00

Jstuelk

76

EBD1A656EE2CBA59E12ED2624758B322F0A2AF06

074501579DF980959E9B85A1859566DB48AC89A9

Biological materials
Mutant
MGNA-A141 (asnB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/141 NBRP B. subtilis, Japan]
References
Reviews
12859215,11395405
Original Publications
10498721,10587437,28317238,36069446

6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617

Page visits: 3363

Time of last update: 2024-03-24 07:19:58

Author of last update: Jstuelk