artR

168

high affinity arginine [wiki|ABC transporter ](ATP-binding protein)

locus: BSU_23960

Molecular weight: 26.80 kDa

pI: 9.13

Protein length: 240 aa

Gene length: 723 bp

function: arginine uptake

product: high affinity arginine [wiki|ABC transporter ](ATP-binding protein)

essential: no

synonyms: artR, artM, yqiZ

List of homologs in different organisms, belongs to COG1126 (Galperin et al., 2021)

Gene

Coordinates: 2,490,574  2,491,296

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 2-236) (according to UniProt)

Structure

• [PDB|4YMS] (from Caldanaerobacter subterraneus, 44% identity) [pubmed|25848002]
• [AF|P54537]

Paralogous protein(s)

• [protein|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN], [protein|B453986691E1231A1C565D469A2A60EBFF2F6BD3|tcyC], [protein|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO], [protein|0D5BC94E21D51373A50D61CE3D0895B3DB28F6B3|pstBB], [protein|0F8048267EC038D8CF673317D969BA27735473A7|yvrO], [protein|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|glnQ], [protein|434BCED6953BE07D592AC229264C5AD0D04CBAF6|pstBA]

[wiki|Localization]

• cell membrane (via [protein|62BD291B91DB0358754957D84A1F3636C247A697|artQ])

Expression and Regulation

Operons

• genes: [gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]

  description: [Pubmed|12107147]

  regulation

  • repressed by casamino acids [Pubmed|12107147]
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]

  regulatory mechanism

  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]

Biological materials

Mutant

• GP1689 Δ([gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR])::''mls'', available in [wiki|Jörg Stülke]'s lab
• GP4156 (Δ[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]::''aphA3'', Δ[gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]::''ermC'', Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::''tet'',), available in [wiki|Jörg Stülke]'s lab
• MGNA-C383 ([gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2381 NBRP B. subtilis, Japan]
• BKE23960 ([gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAATGATTTTGACAGCTTTT,  downstream forward: _UP4_TAGAATATAAAAAAGAAGAA
• BKK23960 ([gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAATGATTTTGACAGCTTTT,  downstream forward: _UP4_TAGAATATAAAAAAGAAGAA

References

• 10092453,11423008,12107147,25848002