argS

168

arginyl-tRNA synthetase, universally conserved protein

locus: BSU_37330

Molecular weight: 62.55 kDa

pI: 5.11

Protein length: 556 aa

Gene length: 1671 bp

function: translation

product: arginyl-tRNA synthetase

essential: yes

ec: 6.1.1.19

synonyms: argS

List of homologs in different organisms, belongs to COG0018 (Galperin et al., 2021)

Gene

Coordinates: 3,833,650  3,835,320

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• ATP + L-arginine + tRNA^Arg --> AMP + diphosphate + L-arginyl-tRNA^Arg (according to UniProt)

Protein family

• [wiki|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)

Structure

• [PDB|3FNR] (from ''Campylobacter jejuni'', 39% identity, 56% similarity)
• [AF|P46906]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• [wiki|universally conserved protein]

Expression and Regulation

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Expression vectors

• pGP2897: expression of Strep-''argS'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

References

• 36815589,9353933,26883633