argC

argC
168

N-acetyl-g-glutamyl-phosphate reductase

locus
BSU_11190
Molecular weight
37.92 kDa
pI
5.17
Protein length
Gene length
function
biosynthesis of arginine
product
N-acetyl-g-glutamyl-phosphate reductase
essential
no
ec
1.2.1.38
synonyms
argC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0002 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,195,034 → 1,196,071
Visit Sartorius.com Visit Sartorius.com
Advertisement
The protein
Catalyzed reaction/ biological activity
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate --> H+ + N-acetyl-L-glutamyl 5-phosphate + NADPH (according to UniProt)
Protein family
NAGSA dehydrogenase family (single member, according to UniProt)
Structure
[PDB|1VKN] (from Thermotoga maritima, 43% identity)
[AF|P23715]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]-[gene|0FF858E44B3C557EE2C8B3749A458F152E1C535C|argJ]-[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]-[gene|7FA5502FE82D55EC176F68B74B24293EC9B7D4D7|argD]-[gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]-[gene|7E6386F1949A6F46E4332BAB8F803742EB076AF0|carB]-[gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]
description
[Pubmed|7511775]
regulation
repressed by arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]) [Pubmed|1312212]
induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
[protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]: repression, [Pubmed|1312212], in [regulon|protein:62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|24843172], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7511775], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]→[gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]

2024-03-28 16:18:25

Jstuelk

70

bd8576e7d6b5ef98ec61ff0587a9708cd8d39155

DA268D208C03E6C5DAF0361E4714668AC025F3EA

Biological materials
Mutant
BKE11190 (Δ[gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11190 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAACAAGTTCACCCTCTT,  downstream forward: _UP4_CCATAGAACGGGAGAGAATC
BKK11190 (Δ[gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11190 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAACAAGTTCACCCTCTT,  downstream forward: _UP4_CCATAGAACGGGAGAGAATC
GP3708 ([gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]::aphA3 trpC2) available in the [wiki|Stülke] lab
Expression vectors
pGP3770: expression of Strep-''argC'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3775: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
pGP3785: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab
pGP3851: expression of ''argC'' (''gapA'' RBS) by [wiki|pBQ200] in ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab
References
6096675,2117746,7511775,12107147,24843172,2497296,1312212

62678D45FA1CB1B6AB801FB9F574DDE10DEE068D

Page visits: 4845

Time of last update: 2024-03-28 07:43:30

Author of last update: Robert.warneke