argB

168

N-acetylglutamate 5-phosphotransferase

locus: BSU_11210

Molecular weight: 27.57 kDa

pI: 6.21

Protein length: 258 aa

Gene length: 777 bp

function: biosynthesis of arginine

product: N-acetylglutamate 5-phosphotransferase (a.k.a. acetylglutamate kinase)

essential: no

ec: 2.7.2.8

synonyms: argB

List of homologs in different organisms, belongs to COG0548 (Galperin et al., 2021)

Gene

Coordinates: 1,197,326  1,198,102

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The protein

Catalyzed reaction/ biological activity

• ATP + N-acetyl-L-glutamate --> ADP + N-acetyl-L-glutamyl 5-phosphate (according to UniProt)

Protein family

• acetylglutamate kinase family (single member, according to UniProt)

Structure

• [PDB|2BTY] (from Thermotoga maritima, 45% identity) [pubmed|16376937]
• [AF|P68729]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• GP2481 ([gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]::''ermC''), available in [wiki|Jörg Stülke]'s lab
• GP2856 ([gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
• BKE11210 (''[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]''::''ermC'') (available in the BGSC and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE11210 ([gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCTTCATTTCGCTCCCCT,  downstream forward: _UP4_GTCAAAGCAAAGGAGGCTGT
• BKK11210 ([gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCTTCATTTCGCTCCCCT,  downstream forward: _UP4_GTCAAAGCAAAGGAGGCTGT

Expression vectors

• pGP3010: expression of Strep-''argB'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
• pGP3011: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab
• pGP3012: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
• pGP3854: expression of ''argB'' (''gapA'' RBS) by [wiki|pBQ200] in ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

References

• 6096675,24843172,12107147,16376937,1312212,7511775