araM

168

glycerol-1-phosphate dehydrogenase, L-arabinose operon

locus: BSU_28760

Molecular weight: 42.90 kDa

pI: 5.63

Protein length: 394 aa

Gene length: 1185 bp

function: biosynthesis of phosphoglycerolipids

product: glycerol-1-phosphate dehydrogenase

essential: no

ec: 1.1.1.261

synonyms: araM, yseB

List of homologs in different organisms, belongs to COG0371 (Galperin et al., 2021)

Gene

Coordinates: 2,943,006  2,944,190

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The protein

Catalyzed reaction/ biological activity

• NAD⁺ + sn-glycerol 1-phosphate --> dihydroxyacetone phosphate + H⁺ + NADH (according to UniProt)
• NADP⁺ + sn-glycerol 1-phosphate --> dihydroxyacetone phosphate + H⁺ + NADPH (according to UniProt)

Protein family

• glycerol-1-phosphate dehydrogenase family (single member, according to UniProt)

[wiki|Cofactors]

• NADH (according to UniProt)

Structure

• [AF|P94527]

[wiki|Localization]

• cytoplasm (according to UniProt)

Biological materials

Mutant

• MGNA-A985 (araM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/985 NBRP B. subtilis, Japan]
• BKE28760 ([gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTGAACGTCAGCTGCGATAC,  downstream forward: _UP4_TAGCCCGCACCTCGAATGGA
• BKK28760 ([gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTGAACGTCAGCTGCGATAC,  downstream forward: _UP4_TAGCCCGCACCTCGAATGGA

References

• 18558723,9084180,10417639,12949161,9084180