araA

168

L-arabinose isomerase

locus: BSU_28800

Molecular weight: 56.37 kDa

pI: 5.47

Protein length: 496 aa

Gene length: 1491 bp

function: arabinose utilization

product: L-arabinose isomerase

essential: no

ec: 5.3.1.4

synonyms: araA

List of homologs in different organisms, belongs to COG2160 (Galperin et al., 2021)

Gene

Coordinates: 2,947,385  2,948,875

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The protein

Catalyzed reaction/ biological activity

• L-arabinose --> L-ribulose (according to UniProt)

Protein family

• arabinose isomerase family (single member, according to UniProt)

Structure

• [PDB|2AJT] (from ''Escherichia coli'', 51% identity, 71% similarity)\t[Pubmed|16756997]
• [AF|P94523]

Kinetic information

• k (cat) of 14,504 min(-1) and  k (cat)/K (m) of 121 min(-1) mM(-1) for L-arabinose [Pubmed|19727704]

Expression and Regulation

Operons

• genes: [gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]-[gene|E0525A4187D9C8A23F23BC8F883973896C8D295D|araB]-[gene|3859854178E573AE8ACCE6DF85E32C495D316DA1|araD]-[gene|BBAD1638F435791C1846EFFAD6B6F82CE491FDF1|araL]-[gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]-[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]-[gene|8AF43CA410F3381209196E1B9A4FE66D78020793|araP]-[gene|AE532A8931D516CC676E765D1D1293E73C335D23|araQ]-[gene|2B2629D275491A9F7031D5A99BAA001B011A765B|abfA]

  description: [Pubmed|9084180]

  regulation

  • induced by arabinose ([protein|search|AraR]) [Pubmed|10417639]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12949161], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|A567466894AE9DE7CDE7816615433A37532297B5|araR]: repression, [Pubmed|10417639], in [regulon|protein:A567466894AE9DE7CDE7816615433A37532297B5|araR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9084180], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• BKE28800 ([gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAAACTGCCCCTTTCT,  downstream forward: _UP4_CTTCAAAAGTAGAGGGGGAT
• BKK28800 ([gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAAACTGCCCCTTTCT,  downstream forward: _UP4_CTTCAAAAGTAGAGGGGGAT

References

• 16585763,11418559,9084180,12949161,2500424,10417639,12949161,9084180,19727704