melD

168

ABC transporter subunit for melibiose and raffinose (permease)

Locus: BSU_30280

Molecular weight: 34.01 kDa

Isoelectric point: 9.08

Protein length: 303 aa

Gene length: 912 bp

Function: uptake of melibiose and raffinose

Product: ABC transporter (permease)

Essential: no

Synonyms: melD, amyD

Genomic Context

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List of homologs in different organisms, belongs to COG1175 (Galperin et al., 2021)

Gene

Coordinates: 3,099,123  3,100,034

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 79-291) (according to UniProt)

Structure

• 4TQU (PDB) (from Sphingomonas sp., 30% identity) PubMed
• O34706 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: melE-melD-melC-melA

  Description: PubMed

  Regulation

  • repressed by glucose (CcpA) PubMed

• Genes: melR-melE-melD-melC-melA

  Description: PubMed

  Regulation

  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • MelR: repression, PubMed, in melR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A799 (amyD::erm), available at the NBRP B. subtilis, Japan
• BKE30280 (melD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCACATGAACGTCCCTTG,  downstream forward: _UP4_AAAAGAAAGGAGACGGAAAT
• BKK30280 (melD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCACATGAACGTCCCTTG,  downstream forward: _UP4_AAAAGAAAGGAGACGGAAAT

References

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