adhB

168

forespore-specific protein, similar to alcohol dehydrogenase

locus: BSU_26970

Molecular weight: 41.04 kDa

pI: 5.66

Protein length: 378 aa

Gene length: 1137 bp

function: unknown

product: unknown

essential: no

synonyms: adhB

List of homologs in different organisms, belongs to COG1063 (Galperin et al., 2021)

Gene

Coordinates: 2,753,452 → 2,754,588

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The protein

Protein family

• [wiki|zinc-containing alcohol dehydrogenase family] (according to UniProt)

Structure

• [PDB|4CPD] (from Thermus Sp., 34% identity)
• [AF|O06012]

Modification

• CoA is attached to Cys-150 in spores [pubmed|33206970]

Paralogous protein(s)

• [protein|7F4C5AD47D167683F96CC2841039512E70CC4F1C|yycR], (33%),alcohol dehydrogenase

[wiki|Localization]

• spore wall (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|7D61DB3A298C253BBC1C7B3AE169F8AAAE4610BB|yraG]-[gene|30A4306899B586898BC5B3666AD43D9DEE0579E1|yraF]-[gene|EA4CC0D36C39957DBBF9B1B11F65F33E8CD81FFE|adhB]-[gene|1D9D20DA24C13EF450710E8EDC5A36311DAF1BF0|yraE]-[gene|0D99D9ECB3BCD6E3F58F8CE8E8CC5AF718C1ECD8|yraD]

  description: [Pubmed|16497325]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16497325]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|16497325], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• MGNA-A261 (adhB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/261 NBRP B. subtilis, Japan]
• BKE26970 (Δ[gene|EA4CC0D36C39957DBBF9B1B11F65F33E8CD81FFE|adhB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTGTAAAACCTCCAGT,  downstream forward: _UP4_CCTTAAAAAACGGAGGTTAC
• BKK26970 (Δ[gene|EA4CC0D36C39957DBBF9B1B11F65F33E8CD81FFE|adhB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTGTAAAACCTCCAGT,  downstream forward: _UP4_CCTTAAAAAACGGAGGTTAC

References

• 16497325,33206970