acpA

168

acyl carrier protein

Locus: BSU_15920

Molecular weight: 8.45 kDa

Isoelectric point: 3.59

Protein length: 77 aa

Gene length: 234 bp

Function: fatty acid biosynthesis

Product: acyl carrier protein

Essential: yes

E.C.: null

Synonyms: acpA, acpP

Genomic Context

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List of homologs in different organisms, belongs to COG0236 (Galperin et al., 2021)

Gene

Coordinates: 1,665,337  1,665,570

Phenotypes of a mutant

• essential PubMed

The protein

Protein family

• acyl carrier protein (ACP) family (single member, according to UniProt)

Domains

• Carrier domain (aa 2-77) (according to UniProt)

Structure

• 1F80 (PDB) (complex with AcpS) PubMed,  3EJE (PDB) (complex with BioI) PubMed,  1HY8 (PDB)
• P80643 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot), during sporulation in the mother cell PubMed

Expression and Regulation

Operons

• Genes: acpA

  Description: PubMed

  Regulatory mechanism

  • FapR: repression, PubMed, in fapR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: fapR-plsX-fabD-fabG-acpA

  Description: PubMed

  Regulation

  • ''FapR'': repressed in the absence of malonyl-CoA or malonyl-ACP (FapR) PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • FapR: repression, PubMed, in fapR regulon
  • ComA: activation, (ComA) PubMed, in comA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Expression vectors

• pGP2893: expression of Strep-acpA by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Labs working on this gene/protein

• Diego de Mendoza, Universidad Nacional de Rosario, Argentine homepage

References

Reviews

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Original Publications

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