At Gram+ 2022 in Prague, there will be a workshop on how to get the most out of SubtiWiki. We will present newly added features. Moreover, we hope to learn from you what you would like to have added to SubtiWiki to improve it!



  • [wiki|C minimal medium]
  • [wiki|M9 minimal medium]
  • [wiki|Spizizen minimal medium]

A collection of mutant strains

The complete mutant strain collection from the Koo et al. paper is now available: get access here.

Toolboxes for the work with B. subtilis

  • the biobrick box PubMed
  • the biobrick box 2.0 PubMed
  • a part toolbox to tune genetic expression PubMed

Analysis of protein-protein interactions

  • Bacterial Two Hybrid: [wiki|BATCH]
  • [wiki|SPINE]: A method to detect in vivo [wiki|Protein-protein interactions|protein-protein interactions]
  • in vivo detection of protein-protein interaction using [protein|search|DivIA] and GFP  PubMed

Analysis of membrane proteins

application of a split green fluorescent protein reporter PubMed


List of available [wiki|Antibodies|antibodies]

Protein expression

the LIKE system PubMed

Strain construction

  • introduction of markerfree deletions: PubMed
  • generation of markerfree mutations: PubMed
  • an improved method for transformation: PubMed

RNA analysis

Mechanical cell disruption and extraction of RNA from B. subtilisMedia:SOP-RNA.pdf

Genetik work with non-standard strains

  • transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
  • efficient electroporation of B. subtilis PubMed
  • a novel transformation protocol for B. subtilis DB104 PubMed

General procedures

Standard operation procedures ([wiki|SOPs]) of the SYSMO-BACELL consortium

Microscopic techniques

  • Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed
  • Assay of gene expression dynamics using live cell imaging PubMed
  • Visualization and quantification of gene expression heterogeneity in growing microbial cells PubMed
  • GFP variants specifically optimizd for use in B. subtilis PubMed
  • Quantitative analysis of cell types by comparing microscoy images PubMed
  • Semi-automated single cell analysis using membrane or DNA staining PubMed

Transposon mutagenesis

mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed

Protein-Dna interactions

ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed

Genomic engineering

  • generalized bacterial genome editing using mobile group II introns and Cre-lox PubMed
  • genome engineering using a synthetic gene circuit PubMed
  • Ordered Gene Assembly in Bacillus subtilis (OGAB) PubMed
  • construction of a super-competent strain PubMed
  • use of the CRISPR-Cas system PubMed
  • A review on genome engineering: PubMed

Visual descriptions of methods of B. subtilis in the Journal of Visual Experimentation

<pubmed> 24473333 24457605 24300024 24300445 22371091 20142799 21841760 </pubmed>

Key references

<pubmed>22179594 20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626 23956387 22285978,22113911 24002656 24282588 26238998 25990947 26732353</pubmed>


Page visits: 933

Time of last update: 2022-05-19 06:25:53

Author of last update: Bzhu