ansA

ansA
168

L-asparaginase

locus
BSU_23580
Molecular weight
36.30 kDa
pI
4.45
Protein length
Gene length
function
asparagine degradation
product
L-asparaginase
essential
no
ec
3.5.1.1
synonyms
ansA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0252 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,455,819 → 2,456,808
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The protein
Catalyzed reaction/ biological activity
H2O + L-asparagine --> L-aspartate + NH4+ (according to UniProt)
Protein family
asparaginase 1 family (with [protein|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ], according to UniProt)
[wiki|Domains]
Asparaginase/glutaminase domain (aa 2-322) (according to UniProt)
Structure
[PDB|5OT0] (from Thermococcus kodakarensis, 43% identity) [pubmed|29095161]
[AF|P26900]
Paralogous protein(s)
[protein|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ]
Expression and Regulation
Operons
genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]
description
[Pubmed|1711029]
regulation
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: repression, [Pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1711029], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]→[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]

2024-03-23 21:01:44

Jstuelk

57

26B0E8E9928B9258DD0E99A3A3CCC1B9E0B8F068

0371500E4D8DAAD3D8BE963EF8C39327F0863D20

genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
[protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]: expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: transcription repression, [pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
Open in new tab

[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]→[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]

2024-03-23 21:02:52

Jstuelk

63

6E6CC6ADE2F20EBE93C9F1ED4C2EF4E87AF126B1

D7F0E184034763E326DE2841E35068D2FD73DD5D

Biological materials
Mutant
GP1153 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''ermC'') available in [wiki|Jörg Stülke]'s lab
GP4256 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''cat'') available in [wiki|Jörg Stülke]'s lab
BKE23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC,  downstream forward: _UP4_TAAAATGTAAAAACGATAAC
BKK23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC,  downstream forward: _UP4_TAAAATGTAAAAACGATAAC
lacZ fusion
pGP872 (in [wiki|pAC5]), available in [wiki|Stülke] lab
References
8478318,1711029,11914346,22383849,29095161,33782055,34293360,38483038

FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD

Page visits: 8159

Time of last update: 2024-03-28 12:25:46

Author of last update: Jstuelk