pbpB

pbpB
168

class B penicillin-binding protein 2B, Pbp2B, works together with FtsW in the divisome in septal cell wall synthesis

Locus
BSU_15160
Molecular weight
79.12 kDa
Isoelectric point
9.23
Protein length
Gene length
Function
septation, cell division
Product
penicillin-binding protein 2B, member of the divisome
Essential
yes
Synonyms
pbpB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,581,947 → 1,584,097
Phenotypes of a mutant
essential PubMed
deletion of the PASTA domains is possible, results in impaired cell division and heat sensitivity PubMed
The protein
Catalyzed reaction/ biological activity
septal cell wall synthesis (this function can be taken over by PBP3) PubMed
assembly of the cell division complex (this is the reason for PbpB essentiality) PubMed
Protein family
transpeptidase family (according to UniProt)
contains two C-terminal PASTA domains (aa 598-657, aa 658-714) (according to UniProt)
Structure
1RP5 (PDB) (PBP 2x from Streptococcus pneumoniae, 32% identity) PubMed
Paralogous protein(s)
cell membrane PubMed
localizes to the division site, this depends on FtsW PubMed
extracellular (signal peptide) PubMed
localizes to the sporulation septum during sporulation PubMed
during vegetative growth: septal PubMed
Expression and Regulation
Operons
Description
Regulation
constitutively expressed PubMed
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mraZpbpB

2025-03-26 00:21:37

Jstuelk

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d5321fe6b1e9387ca670f35171261c39867c1f3d

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Biological materials
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
FLAG-tag construct
GP2003 pbpB-3xFLAG spec (based on pGP1331), available in Jörg Stülke's lab
GFP fusion
3122 trpC2 pbpB::pSG5061 (cat Pxyl-gfpa-pbpB1-825) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC (due to essentiality of pbpB, the strain is xylose-dependent)
Labs working on this gene/protein
Jeff Errington, Newcastle University, UK homepage
References
Reviews
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Original Publications
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Time of last update: 2025-04-08 20:28:35

Author of last update: Jstuelk