pdhD
168
dihydrolipoamide dehydrogenase E3 subunit of both pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes
locus
BSU_14610
Molecular weight
49.57 kDa
pI
4.76
function
links glycolysis and TCA cycle, enzyme in TCA cycle
product
dihydrolipoamide dehydrogenase E3 subunit of both pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes
essential
no
ec
1.8.1.4
synonyms
pdhD, citL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1249 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,531,870 1,533,282
Phenotypes of a mutant
defects in sporulation and unable to grow on glucose as single carbon source [Pubmed|11976308]
The protein
Catalyzed reaction/ biological activity
Protein N6-(dihydrolipoyl)lysine + NAD+ --> protein N6-(lipoyl)lysine + NADH2 (according to UniProt)
Protein family
class-I pyridine nucleotide-disulfide oxidoreductase family (with [protein|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL] and [protein|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV], according to UniProt)
[wiki|Cofactors]
FAD (according to UniProt)
Structure
[PDB|1EBD] (complex with binding domain of dihydrolipoamide acetylase, Geobacillus stearothermophilus), [PDB|1EBD] (complex with binding domain of dihydrolipoamide acetylase, ''Geobacillus stearothermophilus'')
[AF|P21880]
Modification
phosphorylated (Ser/Thr/Tyr) [Pubmed|17726680]
Effectors of protein activity
Inhibited thiamine 2-thiothiazolone diphosphate and NADH [Pubmed|6414463]
Low sensibility to NADPH [Pubmed|6414463]
Kinetic information
Michaelis-Menten [Pubmed|6414463]
Paralogous protein(s)
[protein|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV], [protein|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL]
[wiki|Localization]
cytoplasm (according to UniProt)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]-[gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]-[gene|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]-[gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]
description
[Pubmed|11976308]
regulation
''[protein|search|pdhA]'': expression activated by glucose (1.9-fold) [Pubmed|12850135]
regulatory mechanism
stringent response: negative regulation, due to presence of guanine at 1 position of the transcript [Pubmed|20081037], in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|20081037], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]-[gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]
description
[Pubmed|11976308]
regulation
''[protein|search|pdhA]'': expression activated by glucose (1.9-fold) [Pubmed|12850135]
Biological materials
Mutant
BKE14610 ([gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGGGAAATCTCCTACTACCA, downstream forward: _UP4_TAATTTTCATATCAAAAACA
BKK14610 ([gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGGGAAATCTCCTACTACCA, downstream forward: _UP4_TAATTTTCATATCAAAAACA
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab
FLAG-tag construct
GP1427 (spc, based on [wiki|pGP1331]), available in the [wiki|Jrg Stlke]'s lab
lacZ fusion
pGP723 (in [wiki|pAC5]), available in [wiki|Jrg Stlke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-26 21:20:02
Author of last update: Jstuelk