ylaN

ylaN
168

unknown

locus
BSU_14840
Molecular weight
10.60 kDa
pI
4.61
Protein length
93 aaSequenceBlast
Gene length
282 bpSequenceBlast
function
unknown
product
unknown
essential
yes
synonyms
ylaN
Outlinks
BsubCyc SubtiList UniProt STRING Expression Browser PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4838

This gene is a member of the following regulons

Gene
Coordinates
1,552,412 → 1,552,693
Phenotypes of a mutant
essential [Pubmed|12682299], the mutant is viable if iron(III) is added to the medium [Pubmed|27238023]
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The protein
Protein family
UPF0358 family (single member, according to UniProt)
Structure
[PDB|2ODM] [Pubmed|17469204]
[AF|O07638]
Expression and Regulation
Operons
genes
[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]
description
[Pubmed|22383849]
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[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]

2023-03-09 09:43:50

ghost

31

9c386f35d52a9a5b91f355bfd78e7c060acf46ba

86720CC44AA5A190E597707744A4AD4E5A7A2AEA

Biological materials
Mutant
GP3324 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]::''cat''), available in [wiki|Jörg Stülke]'s lab
GP3321 (Δ[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::''mls'' Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE14840 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC,  downstream forward: _UP4_TAAATGATAAAACTCAAACT
BKK14840 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|ylaN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC,  downstream forward: _UP4_TAAATGATAAAACTCAAACT
Expression vectors
IPTG inducible expression of Strep-''ylaN'' in ''E. coli'': pGP2579 (in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
IPTG inducible expression of His-''ylaN'' in ''E. coli'': pGP2583 (in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3897 constitutive overexpression of ''ylaN'', based on [wiki|pBQ200], available in [wiki|Jörg Stülke]'s lab
pGP3900 constitutive expression of Strep-''ylaN'' in ''B. subtilis'', based on [wiki|pGP380], available in [wiki|Jörg Stülke]'s lab
pGP3867 constitutive expression of ''ylaN''-Strep in ''B. subtilis'', based on [wiki|pGP382], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), pGP3868, pGP3870, pGP3872, pGP3874, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP3593 (based on [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Jörg Stülke]'s lab
References
Reviews
36882621
Original Publications
17005971,17469204,22383849,23420519,27238023,28189581,32949815,36815589

E87DFC182D54EE96D4CA57607E734CDFB18E74EF

Page visits: 3408

Time of last update: 2023-03-23 16:11:39

Author of last update: Jstuelk