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accD
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accD
168

acetyl-CoA carboxylase (beta subunit)

locus
BSU_29210
Molecular weight
28.73 kDa
pI
5.34
Protein length
Gene length
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (beta subunit))
essential
yes
ec
6.4.1.2
synonyms
accD, yttI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0777

This gene is a member of the following regulons

Gene
Coordinates
2,988,693 → 2,989,565
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + N6-carboxybiotinyl-L-lysyl-[protein] --> malonyl-CoA + N6-biotinyl-L-lysyl-[protein] (according to UniProt)
Protein family
AccD/PCCB family (with [protein|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE] and [protein|B6A05AD90A4AA553A8CCD8FFF504F5AD204ABAFB|yqjD], according to UniProt)
[wiki|Domains]
CoA carboxyltransferase N-terminal domain (aa 28-290) (according to UniProt)
Structure
[PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], 64% identity) [Pubmed|16460018]
Modification
phosphorylated on Arg-205 [Pubmed|22517742]
[wiki|Localization]
Membrane-proximal (Spotty) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]
description
[Pubmed|22383849]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
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[gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]→[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]

2021-09-08 12:43:04

ghost

8

b652df2692748c745aab0f8ceb0e50c1ffcb2a82

36A9759B4AA4F3ACBABBC425410BB00129DBEA9D

Biological materials
Expression vectors
purification from ''B. subtilis'' with an C-terminal Strep-tag, for [wiki|SPINE], (in [wiki|pGP382]): pGP1724, available in [wiki|Jörg Stülke]'s lab
pGP1026, N-terminal Strep-tag, based on [wiki|pGP172], purification from ''E. coli'', available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1497 (''mls'', based on [wiki|pGP1370]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
15952903,12121720,15968460
Original Publications
15066985,12663926,14651647,16479537,22517742,22383849,16460018,19965770,28579978,28516784,16460018,27471863

E4997526CC534E38413030E90F7A135ECF65DF40

Page visits: 3375

Time of last update: 2021-09-16 14:37:12

Author of last update: Jstuelk