murAA
168
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
locus
BSU_36760
Molecular weight
46.54 kDa
pI
5.45
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
essential
yes
ec
2.5.1.7
synonyms
murAA, murA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0766 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,777,949 → 3,779,259
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
phosphoenolpyruvate + UDP-N-acetyl-α-D-glucosamine --> phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-α-D-glucosamine (according to UniProt)
Protein family
EPSP synthase family (with [protein|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE] and [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB], according to UniProt)
Structure
[PDB|3SG1] (from ''B. anthracis'', 79% identity, 94% similarity)
[AF|P70965]
Paralogous protein(s)
[protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
division septa [pubmed|36649910]
Additional information
[protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA] is subject to [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-dependent degradation upon glucose starvation [Pubmed|14763982,17981983]
Expression and Regulation
Operons
genes
[gene|32CE4F77F08F926152BECAB56E2841E1FFB27600|ywzB]-[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
description
[Pubmed|22383849]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22383849], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|26735940], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
genes
[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
description
[Pubmed|9353933]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]
genes
[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
description
[Pubmed|26735940]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Expression vectors
pRSETA-murAA, Expression of His(6)-MurAA, avalable in [wiki|Ulf Gerth]'s lab [Pubmed|14763982]
pGP2597: (IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
Antibody
avalable in [wiki|Ulf Gerth]'s lab [Pubmed|14763982]
References
Reviews
Original Publications
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Time of last update: 2024-12-23 11:31:15
Author of last update: Jstuelk