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ftsW
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ftsW
168

SEDS family peptidoglycan glycosyltransferase, cell division protein

locus
BSU_14850
Molecular weight
43.57 kDa
pI
9.72
Protein length
Gene length
function
cell division
product
SEDS family peptidoglycan glycosyltransferase
essential
yes
synonyms
ftsW, ylaO

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0772

This gene is a member of the following regulons

Gene
Coordinates
1,552,899  1,554,110
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
principal peptidoglycan glycosyltransferase of the [wiki|divisome] [Pubmed|27525505]
[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)
Protein family
[wiki|SEDS proteins] (shape, elongation, division, and sporulation) [Pubmed|27525505]
SEDS family (with [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA] and [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE], according to UniProt)
Structure
[PDB|6BAR] (from Thermus thermophilus,corresponds to aa 97... 359 34% identity) [pubmed|29590088]
Paralogous protein(s)
[protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA], [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]
[wiki|Localization]
integral membrane protein
localizes to the division site [Pubmed|27895631]
localization depends on [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] [Pubmed|26679002]
Expression and Regulation
Operons
genes
[gene|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW]-[gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]
description
[pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|yofA]: activation, [Pubmed|17526699], in [regulon|protein:6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|yofA regulon]
stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript [Pubmed|20081037], in [regulon|other_regulator:stringent response|stringent response]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|20081037], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW]→[gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]

2021-09-09 08:00:18

Jstuelk

9

42ada7a92499bdc59de3374453c69f643f2221e3

268DF78C8A44483F25E1E096EBF6ED764E5F0CC4

Biological materials
Mutant
MGNA-A563 (ftsW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/563 NBRP B. subtilis, Japan]
FLAG-tag construct
GP2007 ''ftsW-3xFLAG ermC'' (based on [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
26679002,28975672,33709487
Original Publications
17981970,19429628,17526699,21564336,23701187,27525505,27895631,17379809,27895631,29590088,30692671,32152588,33077634,34097787

8AB7D225DBEE6BD695A4A8A2384D2C029B571C57

Page visits: 2668

Time of last update: 2021-09-13 21:30:43

Author of last update: Jstuelk