yqeV
168
tRNA methylthiotransferase
locus
BSU_25430
Molecular weight
51.49 kDa
pI
5.24
function
tRNA maturation
product
tRNA methylthiotransferase
essential
no
synonyms
mtaB, yqeV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0621 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,621,677 2,623,032
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The protein
Catalyzed reaction/ biological activity
modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA [Pubmed|27913733,20472640]
[sulfur carrier]-SH + AH2 + N6-L-threonylcarbamoyladenosine37 in tRNA + 2 S-adenosyl-L-methionine --> 2-methylsulfanyl-N6-L-threonylcarbamoyladenosine37 in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H+ + L-methionine + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
methylthiotransferase family (with [protein|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB], according to UniProt)
[wiki|Domains]
MTTase N-terminal domain (aa 2-114) (according to UniProt)
[wiki|TRAM domain] (aa 372-437) (according to UniProt)
[wiki|Cofactors]
2 Fe-S clusters [Pubmed|20584901]
Structure
[PDB|4JC0] (from ''Thermotoga maritima'', 29% identity) [Pubmed|23542644]
[AF|P54462]
Paralogous protein(s)
[protein|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
description
[Pubmed|9023197]
regulation
induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]
regulatory mechanism
[protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]: repression, [Pubmed|1339421], in [regulon|protein:2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1339421], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-C496 (yqeV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2494 NBRP B. subtilis, Japan]
BKE25430 ([gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTATGGAAAGCAACAGTTG, downstream forward: _UP4_TAACCTAAAAAATCGGTGCA
BKK25430 ([gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTATGGAAAGCAACAGTTG, downstream forward: _UP4_TAACCTAAAAAATCGGTGCA
References
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Time of last update: 2024-04-26 19:57:28
Author of last update: Melvin.boenninger