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eno

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eno
168

enolase, glycolytic/ gluconeogenic enzyme, universally conserved protein

locus
BSU_33900
Molecular weight
46.42 kDa
pI
4.49
Protein length
Gene length
function
enzyme in glycolysis/ gluconeogenesis
product
enolase
essential
Yes
ec
4.2.1.11
synonyms
eno

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0148

This gene is a member of the following regulons

Gene
Coordinates
3,476,555 3,477,847
Phenotypes of a mutant
no growth on LB, requires glucose and malate [Pubmed|23420519]
essential according to Kobayashi et al. on LB [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
(2R)-2-phosphoglycerate --> H2O + phosphoenolpyruvate (according to UniProt)
Protein family
Enolase family (single member, according to UniProt)
[wiki|Domains]
substrate binding domain (366369)
[wiki|Cofactors]
Mg2+
Structure
[PDB|4A3R] [Pubmed|22198292]
[PDB|1W6T] (from ''Streptococcus pneumoniae'') [Pubmed|15476816]
Modification
phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [Pubmed|17218307,16493705,17726680]
Effectors of protein activity
Inhibited by EDTA [Pubmed|25885]
Kinetic information
reversible Michaelis-Menten [Pubmed|25885]
[wiki|Localization]
cytoplasm [Pubmed|16479537,27708634]
membrane associated [Pubmed|18763711]
aggregates at the cel poles of some cells [pubmed|29439991]
exported, this requires a long, unbent helix (from A108 to L126) [Pubmed|15003462,21856851]
Additional information
Enolase is a [wiki|moonlighting proteins|moonlighting protein] [Pubmed|19193632]
There are indications that this enzyme is an octamer [Pubmed|25885]
extensive information on the structure and enzymatic properties of Eno can be found at [http://www.proteopedia.org/wiki/index.php/Enolase Proteopedia]
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
regulation
expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] [Pubmed|11489127]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ymcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [Pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]→[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]

2021-09-29 21:39:52

Jstuelk

11

7b5a04e0f51be88bb9a430514dd0cb9d39ea3a2c

24906FF9311EDEA4AFD94747BD67B2022E642FAB

genes
[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
Open in new tab

[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]→[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]

2021-10-16 15:34:42

Jstuelk

11

c718c56bfee7b3945ef7060e9c50614ecf6b1cd7

D821B77CDDBA7B2EDE01B5B3E2E0A964498A261D

Biological materials
Mutant
GP594 (''eno''::''cat''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
GP698 (''eno''-''[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]''::''cat''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
Expression vectors
pGP1426 (expression of ''[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pGP1500 (expression of ''[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]'' and ''[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pGP563 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab, [pubmed|20389117]
pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
GP1215 (chromosomal ''eno''-''Strep'' fusion, ''spc''), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
FLAG-tag construct
GP1214 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab [pubmed|21803996]
lacZ fusion
see [gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]
Antibody
available in [wiki|Jörg Stülke]'s lab
GFP fusion
pHT315-yfp-eno, available in [wiki|Mijakovic] lab
GP1700 (in [wiki|pBP43]), expression of '' eno-GFP''::''spc'' under the native promoter, available in [wiki|Jörg Stülke]'s lab [Pubmed|27708634]
labs
[wiki|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
References
Reviews
8994873,34066298
Original Publications
23420519,17726680,17218307,12850135,19193632,11489127,8021172,17505547,25885,20572937,15476816,9988532,21803996,22198292,15378759,28516784
Subcellular localization of enolase
16479537,18763711,20497499,15003462,21856851,24642254,27708634,29439991,34417617

67651D5D3122A89F57F9E7D49074C82A8990A277

Page visits: 7164

Time of last update: 2021-10-20 12:22:34

Author of last update: Jstuelk