polA
168
DNA polymerase I, required for repair of UV- or MMS-induced DNA damage, processing of Okazaki fragments
locus
BSU_29090
Molecular weight
98.89 kDa
pI
4.92
function
DNA replication
product
DNA polymerase I
essential
no
ec
2.7.7.7
synonyms
polA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0749 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,973,182 2,975,824
Phenotypes of a mutant
a [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA] [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA] double mutant is not viable (synthetic lethality) [pubmed|17114254]
strongly reduced stationary phase mutagenesis [Pubmed|27399782]
reduced survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]
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The protein
Catalyzed reaction/ biological activity
has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity [Pubmed|16045613]
maturation of Okazaki fragments (together with [protein|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|RNase HIII]) [pubmed|30670546]
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
Protein family
DNA polymerase type-A family (single member, according to UniProt)
[wiki|Domains]
5'-3' exonuclease domain (aa 174-268) (according to UniProt)
3'-5' exonuclease domain (aa 302-470) (according to UniProt)
Structure
[PDB|6VDE] (from Mycobacterium smegmatis, 40.5% identity) [pubmed|32034423]
[PDB|1XWL] (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)
[AF|O34996]
Paralogous protein(s)
[protein|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA], one of the two proteins has to be present for viability [Pubmed|17114254]
[wiki|Localization]
recruited to replication forks after induction of DNA damage [pubmed|31251806]
Expression and Regulation
Operons
genes
[gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]-[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]-[gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]-[gene|E163A282560DB882B81E783E7E95E1960F4AE4D2|ytaG]
description
[pubmed|22383849]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|23396918], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
GP3532 (Δ[gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]::spec), available in [wiki|Jörg Stülke]'s lab
BKE29090 ([gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
BKK29090 ([gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
References
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Time of last update: 2024-04-18 02:23:39
Author of last update: Jstuelk