ybfK
168
carboxylesterase NP, degradation of pyrethroids
locus
BSU_02260
Molecular weight
32.92 kDa
pI
5.14
function
degradation of isoprenoid-containing lipids such as pyrethroids
product
carboxylesterase NP
essential
no
ec
3.1.1.1
synonyms
ybfK, cesB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0596 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
246,658 247,548
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The protein
Catalyzed reaction/ biological activity
carboxylic ester + H2O --> carboxylate + alcohol + H+ (according to UniProt)
Protein family
[wiki|AB hydrolase superfamily] (according to UniProt)
[wiki|Domains]
[wiki|AB hydrolase-1 domain] (aa 57-284) (according to InterPro)
Structure
[PDB|4CCY] [Pubmed|24418394]
[AF|O31452]
Paralogous protein(s)
[protein|E5A59EE56092F8042128B19D5E8DEFAE41C78000|nap]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|27FB777AEBCC794A5A768C7FE7D8374BCEA75913|ybfK]-[gene|DE49797486CEC172F912D26A6BF2223190BF5DAD|pssA]-[gene|B2C089DCE51CA0CB11947FCD6F4EFAFF66269829|petA]-[gene|F34915697F3EF05E6A683055F46346C73F25CAFC|psd]
description
[Pubmed|22383849,14762009]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|14762009], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|14762009], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
Biological materials
Mutant
MGNA-B934 (ybfK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1933 NBRP B. subtilis, Japan]
BKE02260 ([gene|27FB777AEBCC794A5A768C7FE7D8374BCEA75913|ybfK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTTCTTTTTCCTCC, downstream forward: _UP4_TAGTAAGGAACATGAAAAGA
BKK02260 ([gene|27FB777AEBCC794A5A768C7FE7D8374BCEA75913|ybfK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTTCTTTTTCCTCC, downstream forward: _UP4_TAGTAAGGAACATGAAAAGA
References
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Time of last update: 2024-04-18 10:42:04
Author of last update: Jstuelk