prkC
168
protein kinase C, fine-tuning of cell growth rates in response to Lipid II in different nutrient conditions, induces [category|SW.4.2.4|Germination] of spores in response to DAP-type, and not to Lys-type cell wall muropeptides, stimulates [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] activity
locus
BSU_15770
Molecular weight
71.69 kDa
pI
4.83
function
regulation of growth and germination in response to muropeptides and Lipid II
product
protein kinase
essential
no
ec
2.7.11.1
synonyms
prkC, yloP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2815 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,651,142 1,653,088
Phenotypes of a mutant
unable to germinate in response to muropeptides [Pubmed|18984160]
reduced growth at high salt [Pubmed|25845974]
The protein
Catalyzed reaction/ biological activity
phosphorylation of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] on Thr-101 to stimulate its activity [Pubmed|26102633]
phosphorylates PrkA on Thr-217 [pubmed|36041628]
phosphorylation of RodZ in response to lipid II abundance [pubmed|36797487]
Protein family
[wiki|protein kinase superfamily] (according to UniProt)
[wiki|Ser/Thr protein kinase family] (according to UniProt)
[wiki|Domains]
contains three C-terminal [wiki|PASTA domain]s (aa 356-424, 425-492, 493-559) (binds muropeptides) [Pubmed|18984160]
[wiki|Protein kinase domain] (aa 11-271) (according to UniProt)
Structure
[PDB|4EQM] (intracellular domain of the ''Staphylococcus aureus'' enzyme, 51% identity) [Pubmed|22701750]
[PDB|3PY9] (entire extra-cellular region of PrkC from ''Staphylococcus aureus'') [Pubmed|21208192]
[PDB|4X3F] (intracellular domain of the ''Mycobacterium tuberculosis'' enzyme, 36% identity, 68% similarity) [Pubmed|25586004]
[AF|O34507]
Modification
phosphorylation on Thr-290 [Pubmed|17218307], autophosphorylation on multiple threonine residues [Pubmed|12842463,20389117]
Effectors of protein activity
activated by muropeptides [Pubmed|18984160]
lipid II [pubmed|36797487]
[protein|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB], [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|divIVA], and [protein|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] are required for stimulation of [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] activity [Pubmed|25845974]
[wiki|Localization]
inner spore membrane [Pubmed|18984160]
membrane [Pubmed|12406230]
division septum [Pubmed|25845974]
Expression and Regulation
Operons
genes
[gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]-[gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]-[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]-[gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpeA]-[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B134 (yloP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1133 NBRP B. subtilis, Japan]
GP576 (spc), OMG302 (aphA3), available in [wiki|Jörg Stülke]'s lab
1A820 ( ''prkC''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A820&Search=1A820 BGSC]
1A963 (no resistance), [Pubmed|12399479], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A963&Search=1A963 BGSC]
1A964 (no resistance), [Pubmed|12399479], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A964&Search=1A964 BGSC]
BKE15770 ([gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAGCACTGATCTTCACC, downstream forward: _UP4_GATGAATAACAAGGAGGGAA
BKK15770 ([gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAGCACTGATCTTCACC, downstream forward: _UP4_GATGAATAACAAGGAGGGAA
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP832, available in [wiki|Jörg Stülke]'s lab
for expression/ purification of the kinase domain from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP849, available in [wiki|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP1001, available in [wiki|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]: pGP825, available in [wiki|Jörg Stülke]'s lab, [pubmed|20389117]
lacZ fusion
pGP829 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Phosphorylation of PrkC
Phsiological role of PrkC
Structure/ biochemistry of PrkC
Expression/ localization of PrkC
Targets of PrkC-dependent phosphorylation
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Time of last update: 2024-11-13 20:03:21
Author of last update: Jstuelk