2017-04-24 15:34:122025-05-31 01:00:41

locus

BSU03300

BSU_03300

geneLength

2415

2418

outlinks

bsu

BSU03300

BSU_03300

The protein

Catalyzed reaction/ biological activity

Ammonium hydroxide + 3 NAD(P)⁺ + H₂O = nitrite + 3 NAD(P)H (according to Swiss-Prot)

2 H₂O + 3 NADP⁺ + NH₄⁺ --> 5 H⁺ + 3 NADPH + nitrite (according to UniProt)

2 H₂O + 3 NAD⁺ + NH₄⁺ --> 5 H⁺ + 3 NADH + nitrite (according to UniProt)

The protein

Protein family

nitrite and sulfite reductase 4Fe-4S domain family (according to Swiss-Prot)

nitrite and sulfite reductase 4Fe-4S domain family (with [[protein|CysI]], according to UniProt)

The protein

Paralogous protein(s)

[[protein|NasB]]

[[this]]

Biological materials

Mutant

1A973 ( ''nasD''::''phleo''), [Pubmed|7868621], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A973&Search=1A973 BGSC]

BKE03300 (Δ[[gene|nasD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGATGATCCGCTCCTT, downstream forward: _UP4_TAATGACAAAAACTATCATT

BKK03300 (Δ[[gene|nasD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGATGATCCGCTCCTT, downstream forward: _UP4_TAATGACAAAAACTATCATT

References

Original publications

12823818, 7868621, 8799114, 9765565, 10972836, 16885456, 21091510, 24214949, 25755103

12823818, 7868621, 8799114, 9765565, 10972836, 16885456, 21091510, 24214949, 25755103, 28439033, 20017214

The protein

Structure

[PDB|3KLJ] (from Clostridium acetobutylicum, corresponds to aa 5 ... 399, 32% identity) [pubmed|20017214]