Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-05-22 13:51:54
locus
BSU07840
BSU_07840
essential
yes
no
outlinks
bsu
BSU07840
BSU_07840
[SW|Categories] containing this gene/protein
[SW|metabolism of signalling nucleotides], [SW|essential genes], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
855,114 -> 859,502
Gene
Phenotypes of a mutant
essential [Pubmed|17114254]
essential according to Kobayshi ''et al.'' [Pubmed|17114254], but a deletion mutant could be constructed [Pubmed|27681641]
The protein
Catalyzed reaction/ biological activity
degrades c-di-AMP two 5′ AMP molecules [Pubmed|27414497]
degrades c-di-AMP two 5′ AMP molecules [Pubmed|27414497]
nucleoside 2',3'-cyclic phosphate + H2O --> nucleoside 3'-phosphate + H+ (according to UniProt)
ribonucleoside 3'-phosphate + H2O --> ribonucleoside + phosphate (according to UniProt)
ribonucleoside 5'-phosphate + H2O --> ribonucleoside + phosphate (according to UniProt)
The protein
Paralogous protein(s)
[[protein|YhcR]]
[[this]]
The protein
[SW|Cofactors]
Mn2
Mn2+ [Pubmed|27414497]
The protein
Structure
[PDB|3GVE]
[PDB|2Z1A] (from Thermus thermophilus, 40% identity)
[PDB|3GVE] (fragment, aa 37 - 374)
The protein
[SW|Interactions]
[[protein|YhcS]]-[[protein|YfkN]] [Pubmed|21800427] (anchoring to the cell wall)
Expression and Regulation
Operon
''yfkN'' [Pubmed|16291680]
Expression and Regulation
Regulation
repressed by glucose (5.2-fold) ([[protein|CcpA]]) [Pubmed|12850135]
expressed under conditions of phosphate limitation ([[protein|PhoP]]) [Pubmed|16291680]
Expression and Regulation
Regulatory mechanism
[[protein|CcpA]]: transcription repression [Pubmed|12850135]
[[protein|PhoP]]: transcription activation [Pubmed|16291680]
Biological materials
Mutant
MGNA-C263 (yfkN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2261 NBRP B. subtilis, Japan]
GP2026 (Δ''yfkN::aphA3''), available in [SW|Jörg Stülke]'s lab [Pubmed|27681641]
MGNA-C263 (yfkN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2261 NBRP B. subtilis, Japan]
BKE07840 (Δ[[gene|yfkN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCAGTTCTCCACCTTTCA, downstream forward: _UP4_TAAAAAAGAGCTGCTCGCTA
BKK07840 (Δ[[gene|yfkN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCAGTTCTCCACCTTTCA, downstream forward: _UP4_TAAAAAAGAGCTGCTCGCTA
References
proteinLength
1462
geneLength
4389
Gene
Coordinates
855,114 → 859,502
The protein
Protein family
5'-nucleotidase family (with [[protein|YhcR]], according to UniProt)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]