Mon Aug 31 2015 13:56:58 GMT+0200 (CEST)2025-05-25 01:56:58
description
"Carbon-flux regulating HPr", formerly known as "Catabolite repression HPr-like protein", control of flux through the harmful methylglyoxal pathway, minor cofactor of the CcpA transcription factor
Carbon-flux regulating HPr, formerly known as Catabolite repression HPr-like protein, control of flux through the harmful methylglyoxal pathway, minor cofactor of the [[protein|CcpA]] transcription factor
locus
BSU34740
BSU_34740
ec
outlinks
bsu
BSU34740
BSU_34740
syns
yvcM
[SW|Categories] containing this gene/protein
[SW|transcription factors and their control], [SW|phosphoproteins]
The protein
Protein family
[[PtsH|HPr]] family
HPr family (with [[protein|PtsH]], according to UniProt)
The protein
Paralogous protein(s)
[[PtsH|HPr]]
[[this]]
The protein
[SW|Domains]
[[PtsH|HPr]] domain (1–85)
Hpr domain (aa 1-85) (according to UniProt)
The protein
Structure
[PDB|2AK7] (dimeric Crh-Ser46-P)
[PDB|1ZVV] ([[protein|CcpA]]-Crh-DNA complex)
[PDB|2RLZ] (dimer)
[PDB|1MU4]
[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=24634 NCBI], dimer
[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=64943 NCBI], [[protein|CcpA]]-Crh-DNA complex
[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI], dimeric phosphor-Crh
[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39567 NCBI]
[PDB|2AK7] (dimeric Crh-Ser46-P) [pubmed|16411239]
[PDB|1ZVV] ([[protein|CcpA]]-Crh-DNA complex)
[PDB|2RLZ] (dimer)
[PDB|1MU4]
The protein
[SW|Interactions]
Crh-[[protein|GapA]] [Pubmed|17142398]
Crh-[[protein|CcpA]] [Pubmed|12972249]
[[protein|HprK]]-Crh [Pubmed|12009882]
[[protein|Crh]]-[[protein|MgsA]] (only non-phosphorylated [[protein|Crh]] interacts with [[protein|MgsA]]) [Pubmed|21992469]
The protein
Additional information
Crh does not possess the phosphorylation site used for [SW|PTS] phosphotransfer (His-15 in [[protein|PtsH]]), it can only be phosphorylated on Ser-46
Crh does not possess the phosphorylation site used for [[protein|PtsI]] phosphotransfer (His-15 in [[protein|PtsH]]), it can only be phosphorylated on Ser-46
Expression and Regulation
Operon
''[[protein|yvcI]]-[[protein|yvcJ]]-[[protein|yvcK]]-[[protein|yvcL]]-[[protein|crh]]-[[protein|yvcN]]'' [Pubmed|9237995]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|16272399]
Expression and Regulation
Regulation
very weak stimuation of expression by citrate and succinate [Pubmed|16272399]
Biological materials
Mutant
GP860 (aphA3) [Pubmed|21992469], QB7097 (spc), available in [SW|Jörg Stülke]'s lab
GP860 (Δ[[gene|crh]]::aphA3) [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
QB7097 (spc), available in [SW|Jörg Stülke]'s lab
BKE34740 (Δ[[gene|crh]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT
BKK34740 (Δ[[gene|crh]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT
Biological materials
Expression vector
pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
see ''[[protein|yvcI]]
see [[gene|nahA]]
Biological materials
two-hybrid system
''[[protein|crh]]'', ''crh(Ser46Asp)'', ''crh(Ser46Ala)'' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
[[gene|crh]], crh(Ser46Asp), crh(Ser46Ala) B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Boris Görke], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]
[SW|Anne Galinier], University of Marseille, France
[SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
References
proteinLength
85
geneLength
258
Gene
Coordinates
3,569,292 3,569,549
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Anne Galinier], University of Marseille, France
[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]