Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-27 13:37:22
locus
BSU32440
BSU_32440
ec
outlinks
bsu
BSU32440
BSU_32440
aminos
MKEQHNALQLMMLGLQHMLAMYAGAILVPLIVGAAIGLNAGQLTYLIAIDLFMCGAATLLQLWRNRYFGIGLPVVLGCTFTAVGPMISIGSTYGVPAIYGAIIAAGLIVVLAAGFFGKLVRFFPPVVTGSVVMIIGISLIPTAMNNLAGGEGSKEFGSLDNVLLGFGVTAFILLLFYFFKGFIRSIAILLGLIAGTAAAYFMGKVDFSEVLEASWLHVPSLFYFGPPTFELPAVVTMLLVAIVSLVESTGVYFALADITNRRLSEKDLEKGYRAEGLAILLGGLFNAFPYTAFSQNVGIVQLSKMKSVNVIAITGIILVAIGLVPKAAALTTVIPTPVLGGAMIVMFGMVISYGIKMLSSVDLDSQGNLLIIASSVSLGLGATTVPALFSSLSGAASVLAGSGIVIGSLTAIALHAFFQTKQPNSADIKT
[SW|Categories] containing this gene/protein
[SW|transporters/ other], [SW|utilization of nucleotides], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
3,331,857 -> 3,333,149
The protein
Protein family
xanthine/uracil permease family (according to Swiss-Prot)
[SW|xanthine/uracil permease family] (according to UniProt)
[SW|Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily] (according to UniProt)
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
cell membrane (according to UniProt)
Expression and Regulation
Operon
''[[protein|pucR]]-[[protein|pucJ]]-[[protein|pucK]]-[[protein|pucL]]-[[protein|pucM]]'' [Pubmed|12823818]
''[[protein|pucJ]]-[[protein|pucK]]-[[protein|pucL]]-[[protein|pucM]]'' [Pubmed|12029039]
Expression and Regulation
[SW|Sigma factor]
''[[protein|pucJ]]'': [[protein|SigA]] [Pubmed|12029039]
Expression and Regulation
Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[protein|TnrA]]) [Pubmed|12823818]
induced in the presence of purine nucleotides (inducer: allantoin) ([[protein|PucR]]) [Pubmed|12029039]
Expression and Regulation
Regulatory mechanism
[[protein|TnrA]]: transcription activation [Pubmed|12823818]
[[protein|PucR]]: transcription activation [Pubmed|12029039]
References
proteinLength
430
geneLength
1293
Gene
Coordinates
3,331,857 3,333,149
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3QE7] (E. coli uracil transporter, 28% identity) [pubmed|21423164]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A936 (yunK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/936 NBRP B. subtilis, Japan]
BKE32440 ([[gene|pucK]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTGTGCTGTTCCTTCATCT, downstream forward: _UP4_ATAAAAACGTAAAGGAAGAT
BKK32440 ([[gene|pucK]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTGTGCTGTTCCTTCATCT, downstream forward: _UP4_ATAAAAACGTAAAGGAAGAT