Thu Feb 09 2017 13:44:08 GMT+0100 (CET)2025-05-27 19:39:44
title
yodM
pgpB
description
minor undecaprenyl pyrophosphate phosphatase, not active under standard conditions
phosphatidylglycerol phosphate (PGP) phosphatase , has minor undecaprenyl pyrophosphate phosphatase activity
locus
BSU19650
BSU_19650
function
unknown
phospholipid biosynthesis
product
minor undecaprenyl pyrophosphate phosphatase
phosphatidylglycerol phosphate (PGP) phosphatase
ec
outlinks
bsu
BSU19650
BSU_19650
[SW|Categories] containing this gene/protein
[SW|cell wall synthesis]
Gene
Coordinates on the chromosome (coding sequence)
2,136,913 -> 2,137,524
Biological materials
Mutant
MGNA-B441 (yodM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1440 NBRP B. subtilis, Japan]
GP3652 Δ[[gene|pgpB]]::cat, available in [SW|Jörg Stülke]'s lab
MGNA-B441 (pgpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1440 NBRP B. subtilis, Japan]
BKE19650 ([[gene|pgpB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
BKK19650 ([[gene|pgpB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
References
proteinLength
203
geneLength
612
Gene
Coordinates
2,136,913 2,137,524
The protein
Catalyzed reaction/ biological activity
1,2-diacyl-sn-glycero-3-phospho-(1ʼ-sn-glycero-3ʼ-phosphate) + H2O --> 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate (according to UniProt)
The protein
Protein family
PA-phosphatase related phosphoesterase family (single member, according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Localization]
cell membrane (integral) [Pubmed|28168443]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Expression and Regulation
additional information
the mRNA is cleaved by [[protein|Rnc|RNase III]] [PubMed|26883633]
Biological materials
Expression vector
pGP3502: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [https://www.thermofisher.com/order/catalog/product/K30001#/K30001 pETSUMOadapt], available in [SW|Jörg Stülke]'s lab
pGP3503: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
pGP3506 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab