yuaF

NfeD2, role in maintaining membrane integrity during conditions of cellular stress, confers (together with [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|FloT]) resistance to cefuroxime

locus: BSU_31020

pI: 4.04

mw: 18.46 kDa

protein length: 174 aa

gene length: 525 bp

function: involved in the control of membrane fluidity

product: NfeD family protein NfeD2

essential: no

synonyms: NfeD2

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,182,015 → 3,182,539

The protein

Catalyzed reaction/ biological activity

• [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]-dependent expression of ''[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]'' and the ''[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]'' operon result in reduced membrane fluidity [Pubmed|21542858,22178969]

Protein family

• NfeD family (with [protein|F6B0D27B434D81B7A680555E4457F266B6206B7D|YqeZ])

Structure

• [PDB|2K14] [pubmed|18696230]

[SW|Localization]

• cell membrane, in focal structures [Pubmed|22753055]

Expression and Regulation

Operons

• genes: [gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]
  description: [Pubmed|9987136]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9987136], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulation

  • expressed upon cell wall stress ([protein|search|SigW]) [Pubmed|9987136]

Biological materials

Mutant

• MGNA-A212 (yuaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/212 NBRP B. subtilis, Japan]
• BKE31020 (Δ[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCCTCATCCTTTCTT, downstream forward: _UP4_TAACATAAAAGGAGGAATTT
• BKK31020 (Δ[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCCTCATCCTTTCTT, downstream forward: _UP4_TAACATAAAAGGAGGAATTT

References

• 18696230,9987136,22753055,27362352