luxS [2019-07-09 10:49:48]

S-ribosylhomocysteine lyase, autoinducer-2 production protein, required for surface spreading motility (sliding) of B. subtilis natto and biofilm formation

locus: BSU30670

pI: 5.17

mw: 17.57 kDa

protein length: 157 aa

gene length: 474 bp

function: methionine salvage

product: S-ribosylhomocysteine lyase

essential: no

ec: 4.4.1.21

synonyms: ytjB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]

Gene

Coordinates: 3,137,495 → 3,137,968

The protein

Catalyzed reaction/ biological activity

• S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione (according to Swiss-Prot)

Protein family

• luxS family (according to Swiss-Prot)

Effectors of protein activity

• subject to feedback inhibition [Pubmed|19258532]

Structure

• [PDB|1JQW] (complex with homocysteine), [PDB|1J98] [Pubmed|11601850]

Expression and Regulation

Operons

• genes: [gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]
  description: [Pubmed|9387221]

  additional information

  • subject to feedback inhibition [http://www.ncbi.nlm.nih.gov/sites/entrez/19258532 PubMed]

• genes: [gene|F942C0F4618FACEF35ACA629355BAC288556E7B6|ytiB]-[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

Biological materials

Mutant

• MGNA-B540 (ytjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1539 NBRP B. subtilis, Japan]
• 1A953 ( ''luxS''::''cat''), [Pubmed|17056751], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A953&Search=1A953 BGSC]
• BKE30670 (Δ[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGGCACTCTCCCCTT, downstream forward: _UP4_TAAAATAGAAAGGACCTTTT
• BKK30670 (Δ[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGGCACTCTCCCCTT, downstream forward: _UP4_TAAAATAGAAAGGACCTTTT

References

• 11553770,19258532,9387221,16740951,26779171,17056751,11601850